Metrohm 850 Professional IC Cation - LP-Gradient - Manual (Page 64)

3.19 Separation column
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850 Professional IC Cation – LP Gradient
each column. You can request detailed information on special IC applica-
tions in the corresponding "Application Bulletins" or "Application
Notes", available in the Internet at
http://www.metrohm.com in the
Applications area or via the Metrohm agent responsible free of charge.
Note
The separation column may only be installed after the initial start-up
(see Chapter 4.1, page 55) of the instrument. Until then, use the
6.2744.040 coupling instead of the guard and separation column.
Connecting and rinsing the separation column
1 Connecting the separation column
Caution
When using the columns, always ensure that these are correctly
inserted corresponding to the flow direction indicated.
￭ Remove stoppers from the separation column.
￭ Connect the lower end of the separation column with a
6.2744.070 short PEEK pressure screw to the outlet capillary of
the guard column (if used), or to the column input capillary .
2 Rinsing the separation column
￭ Place beaker under the outlet end of the separation column.
￭ Set the flow rate of the high pressure pump to the value suitable
for the separation column selected.
￭ Start the high pressure pump and rinse the separation column
approx. 10 minutes with eluent.
￭ Switch off the high pressure pump again.
3 Mounting the separation column
￭ Hang separation column with chip into the column holder.
Note
The iColumns are equipped with a chip on which their operating data is
saved. The chip has to be hooked into the chip holder provided for this
so that the column recognition can function.