LaMotte DC1600 Colorimeter - Manual (Page 15)

AMMONIA NITROGEN ~ HIGH RANGE
NESSLERIZATION METHOD CODE 3642
QUANTITY CONTENTS CODE
30 mL Ammonia Nitrogen Reagent #1 V-4797-G
2 x 30 mL *Ammonia Nitrogen Reagent #2 *V-4798-G
1 Pipet, 1 mL, plastic 0354
*WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for
these reagents. For your safety, read label and accompanying MSDS before using.
Ammonia nitrogen is present in various concentrations in many surface and ground water supplies. Any sudden change in
the concentration of ammonia nitrogen in a water supply is cause for suspicion. A product of microbiological activity,
ammonia nitrogen is sometimes accepted as chemical evidence of pollution when encountered in natural waters.
Ammonia is rapidly oxidized in natural water systems by special bacterial groups that produce nitrite and nitrate. This
oxidation requires that dissolved oxygen be available in the water. Ammonia is an additional source of nitrogen as a
nutrient which may contribute to the expanded growth of undesirable algae and other forms of plant growth that overload
the natural system and cause pollution.
APPLICATION: Drinking, surface, and saline waters; domestic and industrial wastes.
RANGE: 0 – 3.0 ppm Ammonia Nitrogen
METHOD: Ammonia forms a colored complex with Nessler’s Reagent in proportion to the amount of
ammonia present in the sample. Rochelle salt is added to prevent precipitation of calcium or
magnesium in undistilled samples.
SAMPLE HANDLING
& PRESERVATION: Preservation is accomplished by the addition of 2 mL of concentrated H
2
SO
4
at 4°C.
INTERFERENCES: Sample turbidity and color may interfere. Turbidity may be removed by a filtration procedure.
Color interference may be eliminated by adjusting the instrument to 100%T with a sample
blank.
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