Iron – LaMotte DC1600 Colorimeter User Manual
Page 47
IRON
1,10-PHENANTHROLINE METHOD
CODE 3668
QUANTITY
CONTENTS
CODE
15 mL
*Acid Phenanthroline Indicator
*2776-E
5 g
*Iron Reducing Reagent
*2777-C
1
Spoon, 0.1 g, plastic
0699
*WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for
these reagents. For your safety, read label and accompanying MSDS before using.
APPLICATION:
Drinking, surface and saline waters; domestic and industrial wastes.
RANGE:
0 – 6.0 ppm Iron
METHOD:
Ferric iron is reduced to ferrous iron and subsequently forms a colored complex with bipyridyl
for a quantitative measure of total iron.
SAMPLE HANDLE
& PRESERVATION:
The sample container should be cleaned with acid and rinsed with deionized water. Addition
of acid to adjust the sample to pH 2 - 3 will prevent deposition of iron on the container walls.
Samples should be analyzed as soon as possible.
INTERFERENCES:
Strong oxidizing agents, cyanide, nitrite, and phosphates; chromium, zinc in concentrations
exceeding 10 times that of iron; cobalt and copper in excess of 5 mg/L, and nickel in excess of
2 mg/L. Bismuth, cadmium, mercury, molybdate, and silver precipitate phenanthroline.
PROCEDURE
1.
Rinse a clean colorimeter tube (0967)with sample water. Fill to the 10 mL mark with sample.
2.
Select setting 2 on the “Select Wavelength” knob and press the “30 Second Read” button.
3.
Insert the tube into the colorimeter chamber and adjust to 100% T with the “Set Blank” knob. This is the 100% T
blank.
4.
Remove the tube from colorimeter. Use the 0.1 g spoon (0699) to add one measure of *Iron Reducing Reagent
(2777). Cap and invert the tube 15 – 20 times to mix.
5.
Remove the cap and add 6 drops of *Acid Phenanthroline Indicator (2776). Cap and invert the tube 3 – 4 times to
mix reagents. Wait five minutes for maximum color development.
6.
After five minutes, press the “30 Second Read” button and insert the tube into colorimeter chamber. Record %T as
soon as the reading stabilizes.
7.
Consult the calibration chart to determine iron concentration in parts per million (ppm).
DC1600 IRON ~ 1,10-PHENANTHROLINE CALIBRATION CHART
%T
9
8
7
6
5
4
3
2
1
0
90
0.02
0.03
0.05
0.07
0.08
0.10
0.11
0.13
0.15
0.17
80
0.18
0.20
0.22
0.24
0.26
0.28
0.29
0.31
0.33
0.35
70
0.37
0.39
0.41
0.43
0.46
0.48
0.50
0.52
0.54
0.57
60
0.59
0.61
0.64
0.66
0.69
0.71
0.74
0.76
0.79
0.81
50
0.84
0.87
0.90
0.93
0.96
0.99
1.02
1.05
1.08
1.11
40
1.15
1.18
1.21
1.25
1.29
1.32
1.36
1.40
1.44
1.48
30
1.52
1.57
1.61
1.66
1.70
1.75
1.80
1.85
1.91
1.96
20
2.02
2.08
2.14
2.21
2.27
2.34
2.42
2.49
2.57
2.66
10
2.75
2.84
2.94
3.05
3.16
3.28
3.41
3.56
3.71
3.88
0
4.07
4.29
4.53
4.82
5.16
5.58
6.13
47