LaMotte DC1600 Colorimeter User Manual
Page 66
PROCEDURE
A. PREPARATION OF HR REAGENT
1.
Use the 50 mL graduated cylinder to carefully add 45 mL of *Ozone Buffer (3991) to amber glass bottle marked HR
Reagent (0680).
2.
Use the 5 mL transfer pipet (0329) and pump (2-2216) to add 5 mL of Indigo Blue Stock Solution (3989) to the
amber glass bottle. Cap and mix.
NOTE: HR Reagent must be made fresh each week. If reagents are refrigerated, they may be kept up to 3 weeks.
B. DETERMINATION OF OZONE
3.
Use the 1.0 mL transfer pipet (2-2170) and pump (2-2216) to add 1.0 mL of HR Reagent to each of 2 clean
colorimeter tubes (0967).
4.
If chlorine is present add 3 drops Chlorine Inhibitor (3990) to each tube. Cap tubes.
5.
Take one of the prepared colorimeter tubes and sampling apparatus (0681) to sampling site.
6.
Lower end of tubing of sampling apparatus to desired depth. Slowly withdraw and depress plunger several times to
purge syringe and tubing. Slowly withdraw plunger to fill purged syringe.
7.
Remove plastic tubing from syringe. Remove cap from the prepared tube. Place tip of syringe against inside of the
prepared tube. Slowly depress plunger and fill to the 10 mL line and cap. This is the Sample Tube.
NOTE: DO NOT SHAKE OR INVERT THE SAMPLE.
8.
Fill the second prepared tube (0967) to the 10 mL line with ozone free water. This is the Reagent Blank.
9.
Select setting 6 on “Select Wavelength” knob and press “30 Second Read” button.
10. Insert the Reagent Blank into colorimeter chamber and adjust “Set Blank” knob so meter reads 43%T (0.00 ppm).
Remove tube from colorimeter.
11. Press “30 Second Read” button and insert reacted Sample Tube into colorimeter chamber. Record%T as soon as the
reading stabilizes.
12. Consult calibration chart to determine ozone concentration in parts per million (ppm).
DC1600 OZONE CALIBRATION CHART
%T
9
8
7
6
5
4
3
2
1
0
90
80
0.40
0.39
0.38
0.38
0.37
0.36
70
0.35
0.35
0.34
0.33
0.32
0.32
0.31
0.30
0.29
0.28
60
0.27
0.27
0.26
0.25
0.24
0.23
0.22
0.21
0.20
0.19
50
0.18
0.17
0.16
0.15
0.14
0.13
0.12
0.11
0.10
0.08
40
0.07
0.06
0.05
0.04
0.02
0.01
0.00
66