Bio-Rad Trans-Blot® Turbo™ Transfer System - Manual (Page 14)

Unpacking and Setup Instructions
The Trans-Blot Turbo starter kit, catalog #170-4155, includes the main unit, two cassettes (bases and
lids), blot roller, standard power cord, quick start guide, instruction manual, and a mixed assortment of
nitrocellulose and PVDF membranes in mini and midi sizes.
Place the system on a level surface with at least 6 cm of clearance in the back for proper ventilation.
Plug the power cord into the back of the instrument, and then connect it to a standard grounded outlet.
Remove the foam ring separating the instrument and cassettes. Power on the instrument using the
switch on the right side of the unit.
Note: Do not place heavy items on the top of the Trans-Blot Turbo system.
Chemical Compatibility
The Trans-Blot Turbo system and cassette components are not compatible with strong acids or bases,
chlorinated hydrocarbons (for example, chloroform), aromatic hydrocarbons (for example, toluene,
benzene), or acetone. The cassettes and instrument casing can be cleaned with water and a mild
detergent, but do not use abrasives or organic solvents. The stainless steel cathode electrode, which is
housed in the cassette lid, can be cleaned with 7% acetic acid and wiped down with water.
Safety/Cautions/Warning
The following guidelines should be observed and followed when using the Trans-Blot Turbo instrument.
The Trans-Blot Turbo instrument has been tested for operation at 15–31ºC ambient temperature
and 0–95% relative humidity (noncondensing). Operating the unit outside these conditions is not
recommended.
• To ensure adequate cooling, be sure that there is at least 6 cm of clearance behind the unit and that
the fan vent at the rear of the unit is not blocked
• Always connect the unit to a grounded AC outlet using the power cord provided
• Use caution when removing a cassette from the unit after a transfer run. The cassette may be warm
to the touch
• Do not operate in extreme humidity (>95%) or where condensation can affect the internal electrical
circuits of the unit
• Operation of the Trans-Blot Turbo system at temperatures <15ºC is not recommended. However,
the unit can function in a cold environment and can be operated immediately. When returning the
unit to normal conditions, allow it to equilibrate to room temperature before use
• For your safety and for the protection of your Trans-Blot Turbo instrument, Bio-Rad suggests you
routinely clean the instrument in accordance with the enclosed instructions and that you routinely
check the contact pins located at the back of the instrument cavity, as outlined in Appendix A.
• Bio-Rad recommends that you rinse and dry the cassette base following each use of the instrument
by rinsing each cassette, base, and lid in deionized water to remove residual salts and to prevent
salt buildup. Air-dry or use a paper towel to dry the cassette.
• Do not add excess or additional buffer to the transfer cassette at any point prior to running the
instrument.
2 Tech Support: 1-800-424-6723 • www.bio-rad.com
Trans-Blot® Turbo™ Blotting System