Bio-Rad Trans-Blot® Turbo™ Transfer System - Manual (Page 24)

Note: A run can be paused by pressing the corresponding A: STOP or B: STOP Navigation button
during the run. The user has a choice to continue from the time point of the pause, restart the run from
the beginning, or terminate the run (Figure 14.)
When the transfer protocol is complete, the screen will display RUN COMPLETE, and an alarm will be
heard (Figure 15).
3.4.a. Disassembling and Removing the Membrane
Note: Use caution when removing a cassette from the unit after a transfer run. The cassette may be
warm.
1. Remove the cassette from the bay by pulling it straight out of the instrument. The LCD menu screen
will automatically return to the protocol screen that has just been completed. The system is ready
for another run. If a different protocol or combination of gels will be blotted in the next run, refer
to Table 1 (page 7) for the appropriate combinations of gels that can be used in a single run, and
proceed to the Home menu to select the correct protocol.
2. Unlock the cassette by turning the dial counterclockwise to the Unlock position.
3. Disassemble the blotting sandwich and place the membrane in a suitable container. If you are using
a PVDF membrane, place it immediately into a storage solution (for example, deionized water or
blocking or staining solution) as the membrane will quickly dry out. If a PVDF membrane requires
rewetting, dip it in methanol or ethanol until uniformly opaque, then wash with deionized water.
4. Discard the ion reservoir stacks after one use; do not attempt to reuse them.
5. Empty residual liquid from the cassette. If no additional transfer will be performed immediately, rinse
the base and lid of the cassette with deionized water and dry them with a paper towel.
6. Turn off the Trans-Blot Turbo system with the power switch if it is no longer required.
Fig. 14. Trans-Blot Turbo system Terminate Run screen.
Fig. 15. Trans-Blot Turbo Run Complete screen.
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Trans-Blot® Turbo™ Blotting System