Bio-Rad Trans-Blot® Turbo™ Transfer System - Manual (Page 19)

Mini Transfer Pack (Single Mini Gel) Setup
The packaging for the mini transfer pack has the two ion reservoir stacks in the left and right wells of
the tray. The right well contains the stack for the anode (below the gel) and is layered with the transfer
membrane (nitrocellulose or PVDF). The left well contains the stack for the cathode (above the gel)
(Figure 5). Use the finger wells of the tray to access the stacks and easily lift them from the tray. The text
above each finger well signifies the stack location in the cassette. The stack and membrane in the right
finger well, labeled Bottom (+) is placed on the anode in the cassette base. The stack in the left finger
well, labeled Top (–) is placed on top of the transfer gel, closest to the cathode.
Midi Transfer Pack (Single Midi Gel or Two Mini Gels) Setup
The packaging for the midi transfer pack has a tray containing the two ion reservoir stacks placed on
top of each other in the tray. The ion reservoir stack on the top has a tab on the right side and is for
the anode (below the gel). This stack is layered with the transfer membrane. The ion reservoir stack for
the cathode (above the gel) is located below the membrane and anode stack and has a tab on the left
side (Figure 6). Use the finger wells and tabs to access the appropriate stack and lift it from the tray. As
with the mini trays, the text above the finger wells signifies the stack location in the cassette. The top
stack and membrane, with the tab on the right, is labeled Bottom (+) and is placed on the anode in the
cassette base. The stack below the anode stack, with the tab on the left, is labeled Top (–) and is placed
on top of the transfer gel, closest to the cathode. For transferring two mini gels simultaneously, arrange
the gels so that the foot of the gels (low–molecular weight side) face each other on the membrane. The
gels will have to be placed longitudinally on the stack (Figure 11).
Fig. 5. The anode stack with the membrane is on the right, while
the cathode stack is on the left.
Fig. 6. The anode stack with the membrane is on the top while the
cathode stack lies beneath the anode stack and membrane.
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Trans-Blot® Turbo™ Blotting System