Bio-Rad Trans-Blot® Turbo™ Transfer System - Manual (Page 28)

3.8 Optimizing Transfer Conditions
The following techniques, alone or in combination, will increase transfer efficiency:
• Use a low-percentage gel or a gradient gel. High-percentage gels retard protein transfer, especially
with large proteins
• High molecular weight proteins may require increased transfer times, particularly when using thick
gels. Increase the transfer time or power conditions
• With long transfer times or high power conditions, some very low molecular weight proteins may
transfer through the membrane to the lower ion reservoir stack. Use a shorter transfer time or
reduce power conditions for most effecient transfers
• Use the blot roller to remove any air bubbles when assembling the transfer sandwich. Air bubbles
between layers of the assembled sandwich will prevent protein transfer, producing blank spots on
the membrane.
Table 4. Recommended power conditions for transfer using the Trans-Blot Turbo system.
Single Mini Gel Two Mini Gels or One Midi Gel
With Turbo Transfer Packs 25 V, 1.3 A, 7 min 25 V, 2.5 A, 7 min
With Standard Semi Dry Components 25 V, 1.0 A, 30 min
Refer to the Bio-Rad Protein Blotting Guide, bulletin 2895, for more information on optimizing
electrophoretic transfer. The Protein Blotting Guide can be downloaded from our website,
www.bio-rad.com , as a PDF file, or call Technical Support at 1-800-424-6723.
16 Tech Support: 1-800-424-6723 • www.bio-rad.com
Trans-Blot® Turbo™ Blotting System