Bio-Rad Whole Gel Eluter and Mini Whole Gel Eluter - Manual (Page 13)

4. Place the lid on top of the harvester. Note that the lid is keyed to the base for proper align-
ment of harvester needles and collection tubes.
5. Attach the harvester to a house vacuum or other vacuum source in the off position. Insert
the needle array into the harvest ports on the left hand side of the Whole Gel Eluter
(unnumbered side). Tighten the thumb screws on both sides of the needle array so that the
gasket seals against the harvest ports. Note that the needle array is keyed for proper align-
ment.
6. With the vacuum control valve fully open, turn on the vacuum source. Slowly close the
control valve to draw a gentle vacuum with which to aspirate the eluates. Aspiration
should be done slowly to prevent splattering and uneven harvesting. It may be nec-
essary to slightly tilt the eluter toward the collection ports to facilitate complete aspiration
of the eluates.
** Thirty tubes of eluate from the Whole Gel Eluter are collected in the Harvesting Box and fourteen
tubes are collected from the Mini-Whole Gel Eluter.
11
Vacuum control
valve
Harvesting box**