Bio-Rad Whole Gel Eluter and Mini Whole Gel Eluter - Manual (Page 24)

Problem Cause Solution
4. Poorly defined fraction- a. Protein bands are a. Carefully align the
ation of proteins. not parallel to the cutting template with the
elution chambers. dye front and molecular
markers of the gel when
cutting.
5. Running conditions a. Buffer concentration a. Make fresh buffer.
outside recommended or pH is incorrect.
range.
6. Splattering of sample a. Vacuum is too strong. a. Decrease vacuum.
in vacuum harvester.
7. Harvester is collecting a. Vacuum is too strong. a. Decrease vacuum.
uneven sample volumes.
Section 9
Equipment and Accessories
Catalog
Number
Product Description
165-1250 Whole Gel Eluter
165-1251 Whole Gel Eluter with large harvester
165-1255 Mini Whole Gel Eluter
165-1256 Mini Whole Gel Eluter with Mini Harvester
165-1260 Large Harvester
165-1261 Mini Harvester
Consumables
165-1270 Whole Gel Eluter Template
165-1271 Mini Whole Gel Eluter Template
165-1275 Cellophane for Whole Gel Eluter , 25
165-1276 Cellophane for Mini Whole Gel Eluter , 25
165-1277 Sealing Tabs for Whole Gel Eluter , 50
165-1278 Sealing tabs for Mini Whole Gel Eluter , 50
165-1279 Roller , 1
165-1280 Lower Chamber filter paper for Whole Gel Eluter , 75
165-1281 Upper Chamber filter paper for Whole Gel Eluter , 50
165-1282 Lower Chamber filter paper for Mini Whole Gel Eluter , 50
165-1283 Upper Chamber filter paper for Mini Whole Gel Eluter , 50
223-9563 DPTP, non-sterile, style M , 500
223-9523 DPTP, non-sterile, style D , 500
223-9645 Culture tubes, 12 x 75 mm , 5 ml capacity, non-sterile, natural
polypropylene, 2,000
223-9500 Micro Test Tube, 1.5 ml, polypropylene, natural, capless, 500
22