Bio-Rad Whole Gel Eluter and Mini Whole Gel Eluter - Manual (Page 9)

1. Place the base of the eluter on a level surface. Place the leveling bubble in the center of
the base and use the adjustable feet to level the base. Insert the bottom electrode plate in
the depression in the base. Align the electrode with the holes for the screws and electrode
stem.
2. Moisten two or three sheets of precut lower chamber filter paper with elution buffer and
lay them on top of the bottom electrode plate. The mini eluter requires two sheets and
the large eluter requires three sheets of filter paper to seal the bottoms of the elution cham-
bers.
3. Wet a precut sheet of cellophane (or dialysis) membrane and place it on the blotting paper.
Work out all air bubbles with the roller provided.
Wet a precut sheet of cellophane and place it on the precut, wetted filter paper.
Using the roller, work out all air bubbles.
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