Bio-Rad Whole Gel Eluter and Mini Whole Gel Eluter - Manual (Page 6)

2.1 Whole Gel Eluter Components
Base and Lower Electrode
The base forms a stable foundation for the eluter. The recess in it houses the lower elec-
trode, the lower filter paper, the cellophane membrane and the elution chamber core.
Adjustable feet enable leveling of the eluter.
Elution Chamber Core
The elution chamber core is a multi-channel collection vessel which is dropped into the
base plate and screwed down over the filter paper and cellophane membrane. Proper sealing
of the core is important for preventing cross-contamination of the fractions. Proteins eluted
from the gel are held in the individual chambers until they are collected with the vacuum har-
vester or transfer pipet.
Upper Plate/Spring Electrode
Attached to the upper plate is the spring electrode. The electrode makes contact with the
upper filter paper. The tension on the spring holds the slab gel in place and seals the tops of
the elution chambers.
Clamps
The upper plate and base plate are held together by clamps on the sides of the eluter. To
open the clamps of the Whole Gel Eluter, pull the bottom outward as shown in Figure A.
Then disengage the clamp as shown in Figure B. The Mini-Whole Gel Eluter has its clamps
mounted in the opposite direction. To open the clamps of the Mini-Whole Gel Eluter, pull
the top outward. Then disengage the clamp at the bottom. To close the clamps, reverse these
procedures.
A. Step 1
B. Step 2
Opening Whole Gel Eluter Clamps
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