Bio-Rad Whole Gel Eluter and Mini Whole Gel Eluter - Manual (Page 12)

12. Thread the tabs through the cutouts of the upper electrode/clamping assembly. (See dia-
gram below.) Place the upper electrode assembly on top of the chamber, aligning the
holes for the screw heads and the stem of the bottom electrode. Gently push down while
closing the side clamps.
Confirm that the sealing tabs are threaded through the cutouts of the upper
electrode/clamping assembly.
3.3 Running the Whole Gel Eluter
1. Place the lid on top of the assembled eluter so that it is fully enclosed. Note that the lid can
be placed in only one orientation, so that the anode and cathode connections cannot be
reversed.
2. Attach the electrical leads to a suitable power supply with the proper polarity.
3. Apply power to the Whole Gel Eluter to begin the elution. As a safety precaution, do not
exceed the voltage and power limits. See Section 4.2 for specific running conditions.
4. Turn off the power at the end of the elution time. Reverse the polarity of the electrical leads
at the power supply. Apply reverse current to the Whole Gel Eluter for 10–15 seconds to
dislodge proteins bound to the cellophane (or dialysis) membrane.
3.4 Harvesting Eluate
Vacuum Harvesting
1. After elution is complete, turn off the power supply, disconnect the electrical leads and
remove the lid. Do not disassemble the eluter.
2. Remove the harvest port sealing tabs from both sides of the eluter. Grasp the tabs and
gently pull them away from the eluter. Pulling too quickly will disturb the samples. Some
spraying from the neighboring holes is normal and not caused by too much elution buffer.
3. For the large format harvester, load the rack with thirty 12 x 75 mm test tubes. Use four-
teen 1.5 ml microcentrifuge tubes with separate press on or screw on lids for the mini-for-
mat harvester. Tubes with caps attached to them will not fit in the rack.
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Sealing tabs
Upper electrode
clamping assembly