Bio-Rad Experion DNA Analysis Kits User Manual

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5. In the New Run dialog (Figure 3.5), select the number of samples to be analyzed. Though all wells

are filled, the Experion system stops the analysis when it reaches the number of samples entered.

Fig . 3 .5 . New Run dialog . The Experion system stops analysis when it
reaches the number of samples entered.

Do not open the lid of the Experion electrophoresis station until the run is complete. The lid does

not lock. Opening the lid aborts the run.

An “IV Check Error” message indicates the system cannot make electrical contact in one of the
wells. This often means there is a bubble at the bottom of the well. Abort the run, and check the
chip for bubbles or empty wells. Refill the affected well(s), and start the run again.

6. Click Start. The green LED in the center of the front panel on the electrophoresis station blinks, and

the system performs a number of checks: it confirms that a chip has been inserted, that all wells
contain liquid, that electrical connections are made, etc. A calibration counter marks the progress of
these calibrations at the upper right of the screen.

Fig . 3 .4 . Details of the New Run screen . The green dot in the lower right corner indicates that communication
between the electrophoresis station and Experion software has been established.

Experion Automated Electrophoresis System

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