Bio-Rad Experion DNA Analysis Kits - Manual (Page 20)

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3. Examine the separation of at least one sample. Click on the sample name in the project tree or on
the lane in the virtual gel to view the electropherogram, which should have the following features (for
example, Figure 4.3):
n
Two resolved marker peaks
n
Sample peaks located between the upper and lower markers
n
Flat baseline
If the upper and lower marker peaks are not properly assigned, you may need to include or exclude
peaks, or manually set the marker (see Sections 6.2 and 6.3).
4. Evaluate the data analysis performed by Experion software (see Chapter 4, Data Analysis). If
necessary, change the analysis settings and parameters — including quantitation methods — by
following the instructions in Chapters 5 and 6.
Fig . 3 .6 . Separation of the DNA ladder . Top, DNA 1K ladder. Bottom, DNA 12K ladder. Note the flat baseline and well-resolved peaks. All
identified peaks are numbered, and the lower and upper markers are indicated by green asterisks (*).
DNA ladder peaks
Baseline
Upper
marker
Lower
marker
DNA ladder peaks
Baseline
Upper
marker
Lower
marker
Experion Automated Electrophoresis System