Bio-Rad Experion DNA Analysis Kits - Manual (Page 7)

Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com 3
Table 1.1. Experion DNA analysis kit specifications.
Experion DNA 1K Experion DNA 12K
Number of samples 11 11
Sample volume 1 µl 1 µl
Total run and analysis time 40 min 30 min
Sizing range 15–1,500 bp 50–17,000 bp
Resolution 25–100 bp: 5 bp 100–1,500 bp: 10%
100–700 bp: 5% 1,500–12,000 bp: 20%
700–1,000: 10%
Quantitation range (≥100 bp) 0.5–50 ng/µl 0.5–50 ng/µl
Limit of detection 0.1 ng/µl 0.1 ng/µl
Sizing accuracy ±10% ±15%
Sizing reproducibility CV ≤5% CV ≤5%
Quantitation accuracy (in TE) ±25% ±20%
Quantitation reproducibility 1 (in TE) CV ≤20% CV ≤20%
Maximum salt concentration:
KCl or NaCl 200 mM 250 mM
MgCl
2
15 mM 15 mM
1 Applies to inter- and intrachip variation.
1.2 Kit Components
Table 1.2. Components of the Experion DNA analysis kits.
Item Description Volume (per Vial) 10-Chip Kit
DNA chip Microfluidic chips used for DNA separation 10 chips
Cleaning chip Chip used for cleaning electrodes 1 chip
DNA gel Proprietary polymeric sieving matrix 250 µl (DNA 1K) 3 vials
650 µl (DNA 12K) 1 vial
DNA stain Proprietary fluorescent dye 40 µl 1 vial
DNA loading buffer Buffer for sample preparation; contains lower and upper 750 µl 1 vial
markers for alignment of samples to the DNA ladder
DNA ladder DNA standard containing 11 DNA fragments of 25–1,000 bp 20 µl 1 vial
(DNA 1K) or 100–10,380 bp (DNA 12K)
Spin filters Used for filtering reagents during sample preparation 3 filters
1.3 Storage Conditions
Table 1.3. Storage conditions.
Item Storage Shelf Life
Experion reagents 4ºC See expiration date on packaging
Experion chips Ambient See expiration date on packaging
Gel-stain solution (GS, prepared) 4ºC 1 month from filtration; do not refilter
Experion DNA 1K and DNA 12K Analysis Kits