Bio-Rad Experion DNA Analysis Kits - Manual (Page 55)

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Appendix C: Glossary
% Total
Calculation performed by Experion
™ software to
show the percent of the total DNA comprised by a
particular peak; used in purity assessments
All-wells view
Mode of display within the electropherogram view
wherein electropherograms of all wells are shown
Baseline
Line of initial data that serves as the control, or
signal representing no fluorescence
Chip
Microfluidic device used to perform separation
using the Experion system
DNA ladder
DNA standard containing 11 DNA fragments of
100–10,380 bp (DNA 12K) or 25-1,000 bp (DNA
1K); separation data from the ladder are used by
Experion software for size determination of sample
fragments
Electropherogram
Trace recording of signal (fluorescence
intensity) as a function of time; displayed in the
electropherogram view
Electropherogram view
Portion of the Experion software user interface
dedicated to display of electropherogram data
Electrophoresis station
Instrument that houses all the electrical, optical,
and hardware components necessary to perform
separation, staining, destaining, band detection,
and imaging of samples; equipped with a universal
power supply to operate at 100–240 V, 50–60 Hz
Gel priming well
Well of the microfluidics chip used to fill the
microchannels of the chip with gel-stain solution
(GS) during priming
Gel-stain solution (GS)
A mixture of gel matrix and stain; used for sample
separation and detection
Gel view
Portion of the Experion software user interface
dedicated to display of virtual gel data
LabChip
Microfluidic “lab-on-a-chip” technology developed
by Caliper Life Sciences; the Experion automated
electrophoresis system was built on LabChip
technology
Lower marker
DNA fragment included in the Experion DNA
sample buffer and used to normalize the
separation of fragments across all wells in the
chips; for the DNA 1K kit, the lower marker is
15 bp and for the 12K kit it is 50 bp
Microchannels
Fine channels approximately the diameter of a
human hair are used for samples separation in
Experion microfluidic chips
Microfluidic technology (microfluidics)
Multidisciplinary field comprising physics,
chemistry, engineering, and biotechnology that
deals with the behavior, precise control, and
manipulation of microliter and nanoliter volumes
Normalization
Alignment of peak migration times across different
sample wells of the same chip; the lower and
upper markers are used by Experion software for
this purpose
Overlay
Function of Experion software that allows different
electropherograms to be superimposed for easier
comparison
Peak
Signal in an electropherogram generated
and identified by the peak-find settings to be
significantly different from the baseline
Priming
Filling the microchannels of the microfluidic chip
with gel-stain solution in preparation for analysis
Priming station
Instrument used to prepare chips for sample
analysis; applies pressure to the priming well
of the microfluidic chip and fills the network of
microchannels with the gel-stain solution
Project folder
User-defined folder containing multiple run files
comprising a particular project
Experion DNA 1K and DNA 12K Analysis Kits