Bio-Rad Experion DNA Analysis Kits - Manual (Page 56)

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Project tree
Hierarchical tree of all saved projects and the data
files they contain
Quantitation
Determination of the concentration of a DNA
fragment in a sample
Relative quantitation
Method of quantitation in which concentrations
are determined against a single-point internal
standard, such as the upper marker
Results and settings
Portion of the Experion software user interface
dedicated to display of tabulated analysis results
and analysis settings and parameters
Run file
File containing data for a single run
Single-well view
Function of Experion software that allows an
electropherogram from a single well to be viewed
Sizing
Determination of the size, in bp, of DNA fragments
Spin filter
Polypropylene spin tubes with filter membranes
used to remove particulate matter from the
gel-stain (GS) and gel (G) by centrifugation
Tree view
Portion of the Experion software user interface
dedicated to display of the project tree; used to
locate and manipulate data files and organize runs
into projects
Upper marker
DNA fragment included in the Experion DNA
sample buffer and used to normalize separation of
fragments across all wells in the chips; also used
as an internal standard for relative quantitation by
default. For the DNA 1K kit, the upper marker is
1,500 bp, and for the 12K kit it is 17,000 bp
Virtual gel
Densitometric, gel-like image generated from
electropherogram data and displaying peaks as
bands; displayed in the gel view
Vortex station
Instrument used to mix RNA or DNA samples and
loading buffers (supplied in the kits) in the chip
wells; used for nucleic acid applications only
Experion Automated Electrophoresis System