Bio-Rad Personal Molecular Imager™ (PMI™) System User Manual

Page 3

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Table of Contents

Section 1

General Information ......................................................................1

1.1

About this Manual ......................................................................................1

1.2

Safety Information......................................................................................1
1.2.1

General Cautions ...........................................................................1

1.2.2

General Warnings ..........................................................................2

1.2.3

Power Safety Information ...............................................................2

1.2.4

Laser Safety Information ................................................................2

1.2.5

Screen Eraser Safety Information...................................................3

Section 2

Introduction ...................................................................................3

2.1

System Capabilities ...................................................................................3

2.2

System Description....................................................................................3

2.3

Theory of Operation ...................................................................................7
2.3.1

Storage Phosphor Detection Mechanism .......................................7

2.3.2

Data Processing and Analysis........................................................7

2.4

Overview of the Imaging Process...............................................................8
2.4.1

Steps in Storage Phosphor Imaging ...............................................8

Section 3

System Installation .......................................................................9

3.1

Operating Requirements............................................................................9
3.1.1

System Location.............................................................................9

3.1.2

AC Power Requirements..............................................................10

3.1.3

Host Computer Recommendations...............................................10

3.2

Setting Up Your PMI System ...................................................................11
3.2.1

Shipping Check ............................................................................11

3.2.2

Unpacking ....................................................................................11

3.2.3

Electrical and Communication Connections..................................13

3.2.4

Quantity One Software Installation ...............................................13

Section 4

System Operation ......................................................................13

4.1

Starting the Scanner ................................................................................13

4.2

Detailed Operating Procedures ................................................................14
4.2.1

How to Prepare Imaging Screens.................................................14

4.2.2

How to Erase Imaging Screens ....................................................15

4.2.3

How to Prepare Samples .............................................................15

4.2.4

How to Use the Exposure Cassette ..............................................18

4.2.5

How to Scan the Imaging Screen .................................................18

4.3

Scanning Unit Precautions.......................................................................20

Section 5

Care and Maintenance ................................................................20

5.1

Scanner Maintenance ..............................................................................20

5.2

Care for PMI Accessories ........................................................................21
5.2.1

General Care of Imaging Screens ................................................21

5.2.2

Radioactive Contamination Check................................................21

5.2.3

Cleaning Imaging Screens ...........................................................21

5.2.4

Storage of the Imaging Screens ...................................................21

5.3

Exposure Cassette Maintenance .............................................................21

5.4

Screen Eraser Maintenance.....................................................................21
5.4.1

Changing Bulbs............................................................................21

Section 6

Troubleshooting ..........................................................................24

6.1

Factors Affecting Image Quality ...............................................................24

6.2

Problem Solving Guide ............................................................................24

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