Bio-Rad Personal Molecular Imager™ (PMI™) System - Manual (Page 8)

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The PMI system (170-9400) is shipped together with following components as shown on
the Fig. 2.1.
• PMI laser scanner
• Quantity One software for image acquisition and 1D analysis
• Sample tray
• Phosphor screen templates
PMI Laser Scanner
The PMI has an internal laser that emits light at a wavelength of 635 nm. The scanner can
scan at resolutions of 50, 100, 200, and 800 um and has a linear dynamic range that
extends over 4.8 orders of magnitude (1:65536). In contrast, x-ray film has a linear dynamic
range that is limited to only 1.5 orders of magnitude.
For complete phosphor imaging workflow the following accessories should be added to the
PMI system:
1. Storage phosphor imaging screens
2. Sample exposure cassettes
3. Screen eraser
4. Multi-sample tray
(1) Storage Phosphor Imaging Screens
Bio-Rad offers a range of storage phosphor screens to match different user requirements.
Table 2.1 summarizes the key features of each screen.
The screens are composed of a barium fluorobromide matrix doped with europium
(BaFBr:Eu). They can be used with traditional autoradiography cassettes without a darkroom,
are easy to handle, and are used solely for the detection of isotopic emissions. All phosphor
screens are reusable and unharmed by repeated exposure to radioactivity. Screens are
sensitive to ß particles and X-rays. All screens are flexible and easy to handle. Exposure
takes place in standard X-ray cassettes. All phosphor screens require erasure prior to
re-exposure, and their lifetime is extended when they are cared for properly.
Imaging Screen-K
This is a general-purpose screen designed for all common radioisotopic emitters, such as
32 P, 33 P, 35 S, and 14 C. These screens are available in 35 x 43 cm and 20 x 25 cm formats.
Screens are guaranteed for 1 year.
Imaging Screen-K/Tritium
This is a special imaging screen available for imaging 3 H. These screens require special
care and handling and are reusable only if cared for properly. These screens are 29 x 24 cm
and are covered by a 6-month warranty.