Bio-Rad Personal Molecular Imager™ (PMI™) System User Manual
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4.2.4 How to Use the Exposure Cassette
Exposure Cassette-K
Fig. 4.6. Steps in use of the exposure cassette-K.
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To open the cassette, push the two release buttons away from the edge.
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Use a clean cloth the wipe the internal surfaces of the cassette to remove any
contamination.
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Tape the prepared sample with the active surface facing upwards on the grid marked
exposure pad, making sure to align the sample straight and in the correct orientation. It
is recommended that Screen Guard then be taped in place over the sample.
•
Place the Imaging Screen-K directly on top the sample with the phosphor (white) side
facing down onto the sample. It is recommended that the sample be at least 1 cm from
all edges of the screen to prevent possible edge effects.
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Make a note of the sample's upper-left and lower-right grid coordinates for later use in
selecting the scan area.
•
Close the cassette and press until the release button catches (snap sound is heard).
4.2.5 How to Scan the Imaging Screen
Inserting Imaging Screen into the Scanner
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Open the scanner door on the front of the instrument.
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Insert the sample tray if it is not already placed in the scanner.
•
Remove the imaging screen from the exposure cassette and quickly place it on the
sample tray with the phosphor surface facing upwards.
Note: When transferring the screen from the exposure cassette to the scanner ,operators
should minimize the screens exposure to direct light as this may erase some of the collect-
ed signal. Some users choose to dim the room lights during the transfer process.