Bio-Rad Personal Molecular Imager™ (PMI™) System User Manual

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Scanning the Samples

1. Start Quantity One software on computer.

2. Open the scan window. Under the File menu, choose Select and use the layered menu

to identify your application (e.g., for radioisotopes using the imaging screen-K, select
Radio/Screen-K).

3. Choose the scan area that you would like to image.

4. Select the desired resolution setting. The highest resolution is 50 µm and the lowest is

800 µm.

5. Select Acquire to begin scanning.

Note: Refer to the Software Manual for detailed acquisition instructions.

Caution: Do NOT open the door of the scanner while the unit is scanning. This may
terminate your scan prematurely and result in the complete loss of image data.

6. When the scanning is complete open the door of the PMI.

7. To remove the imaging screen simply pull out the sample tray and lift off the screen.

8. Once removed, imaging screens can be immediately erased and reused.

4.3 Scanning Unit Precautions

The scanner should be turned on at least 40 seconds before the host computer to allow
adequate time for initialization. Certain PowerMac configurations, however, must be turned
on before the scanner.

Caution: Do not insert or remove the sample tray during a scan or without first powering
up the PMI scanner, as this may damage the unit's scanning head.

Section 5
Care and Maintenance

5.1 Scanner Maintenance

With regular use the PMI scanner should provide years of trouble-free operation, without
the need for operator maintenance. If you suspect that the PMI system requires servicing,
please contact your local Bio-Rad office.

It is recommended that the casing of the scanner be periodically inspected to verify that no
panels are loose or distorted.

Caution: Do not remove the cover from the PMI system, as this voids the warranty. There
are no user-serviceable components in the scanning unit. Attempting to operate the product
with the cover removed may damage the instrument and expose the operator to laser energy.
The PMI system can only be serviced by Bio-Rad or its trained representatives.

Use only mild, non abrasive and water-based detergents to clean the external surface of
the scanner.

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