Bio-Rad Human MMP and TIMP Assays - Manual (Page 13)

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4. Preparing Buffer and Diluent
Prepare Wash Buffer
1. Bring the 10x stock solution to room temperature.
2. If crystals are present, ensure that they are completely dissolved.
Mix the 10x stock solution by inversion before preparing the 1x
wash buffer.
3. To prepare 1x wash buffer, dilute 1 part 10x stock solution with
9 parts deionized water.
5. Prepare Standards and Controls
General Instructions
n
It is essential to prepare standards and quality controls (if included)
exactly as described in this section. Incorrect preparation may lead to
low signal or variable measurements from plate to plate
n
The product data sheet provided with the standards lists the most
concentrated point on the standard curve (S1). Enter this information
into Bio-Plex Manager
™ software as instructed in section 9
Using the Quality Controls (optional)
A single vial of control is provided with the premixed panels only. Their use
is intended for monitoring the day-to-day quality of assay results.
Selecting a Diluent for Standards and Controls
Refer to Table 4 for recommended diluents based on different sample types.
In order to meet the lot-specific control ranges provided on the product data
sheet, both the standards and controls should be reconstituted in Bio-Plex
®
standard diluent HB. If reconstituting in a different diluent, users will need to
establish/validate their own control ranges or acceptance criteria.