Bio-Rad Human MMP and TIMP Assays - Manual (Page 25)

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Bio-Plex Manager software versions 6.0 and higher contain protocols for
most Bio-Plex
® assays. Choose from available protocols or create a new
protocol. To create a new protocol, select File, then New from the main
menu. Locate and follow the steps under Protocol Settings.
1. Click Describe Protocol and enter information about the assay
(optional).
2. Click Select Analytes and create a new panel. Visually confirm the
selected analytes and proceed to step 3.
a. Click Add Panel in the Select Analytes toolbar. Enter a new
panel name. Select Bio-Plex Pro Assay Magnetic from the
assay dropdown list. If using Bio-Plex Manager version 5.0 or lower,
select MagPlex from the assay dropdown list.
b. Click Add. Enter the bead region number and name for the first
analyte. Click Add Continue to repeat for each analyte in the
assay. Refer to the bead regions in parentheses ( ) listed on the
peel-off label provided with the standards.
For reference, bead regions are shown in Table 13.
c. Click Add when the last analyte has been added and click OK to
save the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click Add All.
e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking Rename Panel and entering a new
panel name.