Bio-Rad Human MMP and TIMP Assays User Manual
Page 2
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Table of Contents
Introduction 1
Principle 2
Kit Contents and Storage
4
Recommended Materials
5
Assay Workflow
6
lmportant Considerations
7
Detailed Instructions
7
1.
1. Plan Plate Layout 8
2.
2. Prepare Instrument 9
3.
3. Prepare Wash Method 10
4.
4. Preparing Buffer and Diluent 11
5
5. Prepare Standards and Controls 11
6. Prepare Samples 13
7.
7. Prepare Coupled Beads 17
8
8. Run Assay 18
9
9. Read Plate 22
Troubleshooting Guide
29
Plate Layout Template
34
Calculation Worksheet
35
Safety Considerations
37
Legal Notices
37
Ordering Information
38
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