Bio-Rad VersaFluor™ Fluorometer - Manual (Page 18)

Fig. 4.12. Sample number reset menu.
Section 5
Operating Instructions
5.1 Quick Guide Procedure
This section describes the typical procedure for taking fluorescence measurements. Menu
options are described in Section 4. A typical sample measurement requires five steps:
Note: The instrument should warm up for at least 20 minutes before taking any read-
ings.
1. Selection and insertion of two optical filters.
Insert the emission and excitation filters into the VersaFluor Fluorometer.
2. Set the gain.
Press the SETUP button on the main menu and set the gain to LOW, MED, or HIGH.
3. Zero the instrument.
Set the range to 00000 by pressing the RANGE button. Adjust the range to 00000. Press
EXIT. Place the baseline sample cuvette in the cuvette chamber and press the SET ZERO
button. When blinking stops the fluorescence display should read 0 ± 5. If not, re-zero the
instrument by pressing the SET ZERO button.
4. Set the range.
Set the range by placing a cuvette containing the highest concentration standard in the
cuvette holder and closing the sample compartment lid. Wait approximately 10 seconds
for the detector to adjust to the light conditions. Press the RANGE button. Adjust the
range to the desired setting. The maximum setting is 19,999 RFU. Press EXIT.
5. Measure the sample.
Place a sample cuvette in the cuvette holder and close sample compartment lid. Wait
approximately 10 seconds for the detector to adjust to the light conditions. Record or
print the RFU value in the fluorescence display. Read all remaining samples.
15
CLOCK LAMP SAMPLE
SETUP
ON SET#1 EXIT