Bio-Rad VersaFluor™ Fluorometer - Manual (Page 20)

Optional: The zero of the instrument can be rechecked by placing the baseline cuvette
(blank) in the cuvette holder. Wait approximately 10 seconds for the detector to adjust to
the light conditions. If the instrument needs to be re-zeroed, press the SET ZERO button.
When blinking stops, the fluorescence display should read 0 ± 5.
4. For multiple standard samples, read the remaining samples. Wait approximately 10 sec-
onds for the detector to adjust to the light conditions. Read or print the RFU number in the
fluorescence display. The value shown on the fluorescence display is a measure of the dif-
ference between the baseline sample and the highest concentration standard.
Note: Fluorescence measurements can be made continuously. It is not necessary to zero
the instrument before each measurement unless the baseline conditions change. Check
the zero of the instrument with the baseline fluid (blank) when switching between gain set-
tings.
5.4 Reading Unknown Samples
1. Place a cuvette with an unknown sample in the cuvette holder and close the sample com-
partment lid. Wait approximately 10 seconds for the detector to adjust to the light condi-
tions. Read or print the RFU number in the fluorescence display.
2. Repeat for all remaining unknown samples.
Note: Fluorescence measurements can be made continuously. It is not necessary to zero
the instrument before each measurement unless the baseline conditions change. Check
the zero of the instrument with the baseline fluid (blank) when switching between gain set-
tings.
3. The sample concentrations can be determined by comparing its RFU values with the stan-
dard(s).
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