Spectra of tnf- α antigen capture – Bio-Rad ProteinChip Antibody Capture Kit User Manual
Page 10
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© 2007 Bio-Rad Laboratories, Inc.
Spectra of TNF-
α Antigen Capture
Fig. 2. Spectra illustrating the effect of increasing TNF-a quantity on intensity. The molecular mass
of TNF-
α is 17.5 kD. TNF-α quantities range from 130 amol to 532 fmol. The double peak in these
spectra represents equimolar amounts of TNF-a with and without N-terminus methionine.
0.2
0.1
0
-0.1
0.6
0.4
0.2
0
-0.2
0.4
0.2
0
1.0
0.5
0
1.0
0.5
0
3
2
1
0
10
7.5
5.0
2.5
0
20
10
0
1,400
1,600
1,800
20,000
m/z, kD
In
te
n
s
it
y
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