Bio-Rad ProteinChip Antibody Capture Kit - Manual (Page 4)

© 2007 Bio-Rad Laboratories, Inc.
Materials Needed but Not Included
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15 ml conical tubes
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Rocking platform
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Humidity chamber*
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Lab timer
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Ice bucket and ice
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Microcentrifuge tubes
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Calibrated pipets**
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99+% trifluoroacetic acid (TFA)
Optional Materials
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ProteinChip cassette-compatible bioprocessor (catalog #C50-30011)
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MicroMix 5 plate and tube shaker*** (Diagnostic Products Corporation)
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Sonicating water bath
Shipping and Storage
Table 1. Shipping and storage conditions for ProteinChip antibody capture kit components.
Item Shipping Storage
Anti-human TNF-
α
Dry ice –20°C (stable 6 months)
antibody 4°C (stable 2 weeks)
Recombinant human Dry ice –20°C (stable 6 months)
TNF-
α antigen
4°C (stable 2 weeks)
Bovine IgG Dry ice –20°C
Crosslinking reagent Dry ice –20°C
ProteinChip PG20 arrays Ambient with desiccant 4°C with desiccant
Wash buffer Ambient 4°C
PBS Ambient 4°C
Deactivation buffer Ambient 4°C
HEPES buffer Ambient 4°C
ProteinChip SPA EAMs Ambient Ambient or 4°C
* See the ProteinChip SELDI System Applications Guide for detailed instructions on how to create a
humidity chamber if one is not readily available.
** Calibrated pipets will yield more reproducible results.
*** When using a ProteinChip bioprocessor, a MicroMix 5 is recommended.