Bio-Rad ProteinChip Antibody Capture Kit - Manual (Page 9)

© 2007 Bio-Rad Laboratories, Inc.
Appendix A
Immunoglobulin Binding Affinity to Protein G
The information in Table 2 is provided as a guideline and does not guarantee that all
antibodies will behave identically to the information provided in the table.
Table 2: Binding affinity of various immunoglobulins to protein G.*
Species Immunoglobulin Binding Affinity**
Human Total IgG S
IgG
1
S
IgG
2
S
IgG
3
S
IgG
4
S
Mouse Total IgG S
IgG
1
M
IgG
2a
S
IgG
2b
S
IgG
3
S
Rat Total IgG M
IgG
1
M
IgG
2a
S
IgG
2b
W
IgG
2c
S
Bovine Total IgG S
IgG1 S
IgG2 S
Cat Total IgG W
Dog Total IgG W
Goat Total IgG S
IgG
1
S
IgG
2
S
Guinea pig Total IgG W
Horse Total IgG S
IgG
(ab)
NB
IgG
(c)
MB
IgG(T) S
Pig Total IgG W
Rabbit Total IgG S
Sheep Total IgG S
IgG
1
S
IgG
2
S
* Information provided by Pierce.
** W, weak binding; M, medium binding; S, strong binding; NB, no binding.