Bio-Rad ProteinChip Antibody Capture Kit - Manual (Page 8)

© 2007 Bio-Rad Laboratories, Inc.
Step 5: Reading the ProteinChip PG20 Array in a ProteinChip
SELDI Reader
After the EAM has dried on the array, read the array in a ProteinChip SELDI reader.
The spot protocol used to read the array can be created once and saved. The data
collection parameters of the spot protocol are a function of molecular mass and
need to be adjusted for each antigen. See the operation manual for ProteinChip
data manager software for detailed instructions on how to create an optimized spot
protocol. When optimizing the protocol for the detection of TNF-
α antigen
(molecular mass 17.5 kD), set the Mass Range limits from 0 to 200,000 Da, and
the Focus Mass to 17,500 Da.
Ordering Information
Catalog # Description
K10-00005 ProteinChip Antibody Capture Kit, includes antibody, antigen, reagents, controls,
6 ProteinChip PG20 arrays, ProteinChip SPA energy absorbing molecules (EAMs),
instructions
C55-30058 ProteinChip PG20 Array, A–H format
C50-30011 ProteinChip Cassette-Compatible Bioprocessor, includes ProteinChip array forceps,
cassette hold-down frame, 12 blank ProteinChip arrays
C30-00002 ProteinChip SPA Energy Absorbing Molecules (EAMs),
5 mg/vial, 20