Designing a protocol, Standard protocol elements – Dako Autostainer User Manual

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Section 5

Designing a Protocol


Protocol templates allow you to enter the series of steps required to stain a slide and
save the protocol for future use. Low level security does not allow creating or editing
protocol templates.

Standard Protocol Elements

Protocol elements are categories of reagents used to build the protocol outline in the

Protocol Template Design screen. Reagents can be added, deleted and edited by

choosing protocol element categories from the Edit Lists menu item on the

Programming Grid.

Endogenous Enzyme Block

Protein Block

Primary Antibody

Pretreatment

Secondary Reagent (e.g., biotinylated secondary antibodies or link)

Tertiary Reagent (e.g., enzyme-labeled streptavidin or label)

Labeled Polymer (e.g., used in the EnVision visualization systems)

Substrate-Batch

Substrate

Auxiliary (e.g., counterstain)

Autostainer Handbook

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