Dako Autostainer - Manual (Page 44)

6. Select the Protocol Template button and select a protocol template for the run.
7. Select the Use Template button. The Programming Grid appears with the selected
protocol.
8. Select the desired volume and drop zones for either all slides, or on an individual
side basis.
9. Assign specific antibodies and reagents to all slides and select the Next button. The
Slide Layout Map: Program Slides screen appears.
10. Select the Next button and the Reagent Layout Map appears.
11. Load reagents using the Reagent Layout Map. Check water and buffer volume, and
make sure you have enough space for waste.
12. Select the Next button. The Slide Layout Map: Load Slides screen appears.
13. Load slides using the Slide Layout Map: Load Slides screen.
14. Click the Next button.
15. The Set Start Time screen appears. The system calculates the run time and amount
of buffer and DI water needed to complete the run.
16. Select the Prime Pump (Water) button, and click OK.
17. Select the Prime Pump (Buffer) button.
18. Set reagent levels to Check Volumes or Do Not Check Volumes.
19. Select the Start Run button.
Run Log Screen
During a staining run the Run Log screen is updated with each step performed.
• The
New Program button is used to program a new run while the current run is
in process.
• The Review Program button is used to return to the Programming Grid to view
the run in progress.
• The Emergency Stop button can be used to stop a run at any time.
Shutting Down the Autostainer
1. Select the Sign Off button on the Main Menu.
2. A message prompts, “Quit using Autostainer?” Select the Yes button.
3. Shut down the computer.
36 Autostainer Handbook