Dako Autostainer - Manual (Page 43)

Section 10
Starting a Staining Run
Preparing the System for a Staining Run
After the specimen-containing slides and reagent vials are loaded, perform the following
checklist:
Slides
• Deparaffinized and rehydrated, soaking in buffer containing 0.05% Tween 20
using a squirt bottle to prevent slides from drying while loading additional slides.
• Seated securely in slide rack.
Racks
• Properly seated in the Autostainer.
Reagents
• At room temperature.
• Placed at proper locations in the reagent racks.
• Contain no air bubbles.
• Contain at least the reagent volume in each vial as specified in the Reagent
Layout Map.
Buffer
• Contains at least the volume of buffer indicated in the Reagent Layout Map plus
any additional volume listed for the completion of a delayed run.
Water
• Contains at least the volume of water indicated in the Reagent Layout Map.
Waste Containers
• Have enough room to accommodate the volume of buffer and water used during
the staining run.
Quick Start
1. Turn on the computer system. Double-click the Autostainer icon.
2. Enter User Name and password and press ENTER.
3. Select the Program button on the main menu.
4. Select Slides from the menu bar. (To enter information for a specific slide see
Section 4.)
5. Enter the slide count for the staining run and click OK.
Note: Skip steps six and seven if you are using the default template.
Autostainer Handbook 35