Labconco Biological Safety Cabinets 34610 Series - Manual (Page 46)

Chapter 5: Using The Cabinet
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• Arrange materials to minimize the movement of contaminated
materials into clean areas.
• Keep all discarded contaminated material to the rear of the work area.
• Avoid moving materials or the operator's hands and arms through the
front access opening during use.
• Avoid the use of an open flame. Use disposable lab ware or an
electric incinerator as alternatives.
• Use proper aseptic technique.
• Avoid using techniques or procedures that disrupt the airflow patterns
of the cabinet.
• If there is a spill or splatter during use, all objects in the cabinet
should be surface decontaminated before removal. Thoroughly
disinfect the working area of the cabinet WHILE IT IS STILL IN
OPERATION, to prevent the release of contaminants from the
cabinet.
Final Purging
• Upon completion of work, the cabinet should be allowed to operate
for two to three minutes undisturbed, to purge airborne contaminants
from the work area.
Unloading Materials and Equipment
• Objects in contact with contaminated material should be surface
decontaminated before removal from the cabinet.
• All open trays or containers should be covered before being removed
from the cabinet.
Wipe-Down
• Wipe down the interior surfaces of the cabinet with 70% ethanol, or a
suitable disinfectant, and allow to dry.
• Periodically lift the work surface and wipe down the area beneath it.
• Inspect and clean the towel catch located at the rear of the work area,
beneath the work pan.
• Dispose of rubber gloves appropriately, and have lab coat laundered
properly.
• Wash hands and arms thoroughly with germicidal soap.
Shutdown
• Lower the sash to turn off the fluorescent light and cabinet blower and
activate the UV light if appropriate.