Ivoclar Vivadent CRT bacteria - Manual (Page 2)

7. Allow excess saliva to drip off.
8. Slide the agar carrier back into the vial and close the
vial tightly.
9. Use a waterproof pen to note the name of the patient
and the date on the vial.
10. Place the test vial upright in the incubator, e.g.
Cultura/Ivoclar Vivadent, and incubate at 37 °C / 99 °F
for 48 hours.
11. After removal of the vial from the incubator, compare
the density of the mutans streptococci and lactobacilli
colonies with the corresponding evaluation pictures in
the enclosed model chart.
Tip: Hold the agar carrier slightly oblique under a light
source to facilitate the evaluation.
Modified procedures
A slight modification of the test procedure permits the
determination of the mutans streptococci count in plaque:
• Remove plaque with a fine brush.
• Apply plaque on the blue agar without scratching the
surface. Up to four samples may be applied on the same
carrier in a parallel pattern without any difficulty.
Note: Apply a small amount of water to the NaHCO
3
tablet in case not enough saliva drips onto the tablet.
The preparation of sampling and incubation are carried out
according to the step-by-step procedure described in these
Instructions. As far as the evaluation is concerned, this test
only determines whether or not mutans streptococci are
present.
Evaluation
Findings of 10
5 CFU or more of lactobacilli and mutans
streptococci per ml saliva indicate a high caries risk.
Note
Leaving the test vial in the incubator for an additional day
or two does not influence the number of CFUs.
Disposal
• Do not use new agar carriers that appear to be covered
with microorganisms.
• Before disposing of used agar carriers, disinfect them
with a suitable agent or autoclave them in an
autoclaving bag.
Storage
• Store CRT bacteria in the refrigerator
(2–8 °C / 36–46 °F)
• Do not freeze the material
• Avoid temperature fluctuations
• Protect from light
• Shelf life: see date of expiration
• Do not use CRT bacteria after the indicated date of
expiration
Store out of the reach of children.
For use in dentistry only.
Date information prepared
05/2003
Ivoclar Vivadent AG
FL-9494 Schaan/Liechtenstein
This product has been developed solely for use in dentistry.
Processing and application should be carried out strictly according
to the Instructions for Use. Liability cannot be accepted for damages
resulting from failure to observe the Instructions or the stipulated
area of application. The user is responsible for testing the material
for its suitability and use for any purpose not explicitly stated in
the Instruction. Descriptions and data constitute no warranty of
attributes and are not binding.
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