Luminex xPONENT for MAGPIX IVD - Manual (Page 9)

1. On the Home page, click Probe and Heater under Daily Activities. The Probe &
Heater tab opens.
2. Based on the type of plate you are using, place alignment disks or an alignment sphere
from the Height Alignment Kit in the well.
NOTE: Luminex recommends that you use well D6 (this is the center of a
standard 96-well plate).
• Filter-bottom plate - two (2) 5.08mm disks
• Mylar-bottom plate - two (2) 5.08mm disks
• Conical (V-shaped) plate - one (1) sphere
If you are using a standard 96-well plate, you do not need to use any of the disks or
spheres in the Height Alignment Kit.
3. Ensure that the well location is selected on the plate image. A green pin marks the
selected well.
4. Click Eject to eject the plate carrier.
5. Place the off plate reagent block on the plate carrier. Make sure it is well seated so that it
clips into place.
6. Place a strip well (provided with the Calibration and the Performance Verification kit) in
the off-plate reagent block.
7. In the Strip Wells section, click SD1.
8. Verify that the reservoir is empty.
9. In the Reservoir section, click well RB1.
10. Verify that the plate is not warped. Warped plates can lead to incorrect probe height
adjustment.
11. Place the plate on the plate carrier with well A1 positioned as indicated on the plate
carrier.
12. Click Retract to retract the plate carrier.
13. Type a name for the plate in the Plate Name box.
14. Click Auto Adjust Height. The probe automatically adjusts itself to the locations you
selected.
NOTE: The probe height is automatically set to 0.98mm. The probe
automatically adjusts this distance from the bottom of the plate, or
calibration disks or spheres.
15. Click Eject to eject the plate holder. If you used alignment disks or spheres, remove them
from the plate.
16. Click Save to save the probe height settings for the plate and the well.
NOTE: When you calibrate and save the probe height settings for all three
areas under a plate name, all areas retain the calibration.
CAUTION: Ensure that the probe height is set correctly before calibrating
the system.
For In Vitro Diagnostic Use Starting the MAGPIX System for the First Time
3