Multichannel Systems MEA1060-Up-BC Manual - Manual (Page 14)

MEA Amplifier with Blanking Circuit for Upright Microscopes
14
3.4 Setting Up and Connecting the MEA Amplifier
Warning: Spilled liquid can damage or even completely destroy the electronics of the MEA
amplifier. Please be extremely careful when setting up your perfusion system and when starting
the perfusion. Take care that the flow rates of the inlet and outlet flow match so that flooding
of the amplifier is efficiently prevented.
Note: The housing of the MEA amplifiers was optimized for Zeiss Axiovert microscopes. MEA
amplifiers are compatible to most standard microscopes if the following prerequisites are met.
This MEA amplifier type has been developed for standard upright microscopes with a rectangular
microscope table. The focal plane of the microscope has to be in a distance of 9.5 mm to the
microscope table.
Note: If you use a complete MEA-System, the MEA amplifier will usually be powered by the
isolated power supply (IPS10W) that is integrated into the data acquisition computer. The power
is distributed along the MCS 68-pin MCS high grade cable. If you use your MEA amplifiers
together with a MEA Switch, or if you use a custom data acquisition system
without IPS10W,
you will need an external power supply for operating the amplifier(s). Please ask your local
retailer for more information on setup options.
Note: If you use an USB-ME-System you need an external power supply , for example a PS40W,
for operating the amplifier(s). Please ask your local retailer for more information on setup
options.
1. Connect the MEA preamplifier with blanking circuit to the filter amplifier's input with
a 68-pin MCS standard cable.
Using the MC_Card for data acquisition: Connect the filter amplifier's output to the MC_Card
input labeled MC_Card Ch. 01–64 with a 68-pin MCS standard cable. If you have a MEA120-System,
connect the second MEA amplifier with a 68-pin MCS standard cable to the input for channels
65–128 labeled MC_CX64 Ch. 65–128. If you are using a MEA Switch, connect the amplifiers to
the MEA Switch inputs, and the MEA Switch outputs to the MC_Card.
Using an USB-ME data acquisition device: Connect the filter amplifier's output to the USB-ME
input via 68-pin MCS standard cable. If you are using a MEA Switch, connect the amplifiers to
the MEA Switch inputs, and the MEA Switch outputs to the USB-ME device.
1. Place the MEA amplifier onto the microscope table.
2. Connect the internal heating element to a Temperature Controller's output channel (D-Sub9
socket) with the black cable. (The Temperature Controller is not part of the standard scope of
delivery, but is included in the MEA-System or USB-ME-System, and can also be ordered
separately.) Do not connect the black heating element cable to the data acquisition computer.
3. Connect the serial port of the preamplifier to a free RS232 serial port of the computer, from
which you like to control the MEA amplifier (usually the data acquisition computer) with the
provided RS232 cable. Note that you need a separate Com port for each amplifier in use.
4. Connect up to two analog outputs of the stimulus generator to the two stimulus inputs of the
preamplifier. Connect the corresponding ground to the ground inputs. Please see the illustrations
below for details.
5. Connect a digital Sync Out output of the stimulus generator to the Trigger In input of the
preamplifier with a standard BNC cable. If you use a complete MEA-System, connect the same
Sync Out output to the first digital input bit of the MC_Card. Split the Sync Out output by using
a T connector to connect the devices in serial, but not parallel! Connect the T connector to
the MEA amplifier or to the MC_Card output, but not to the STG output.
6. You should use a Faraday cage or appropriate materials, for example aluminum foil, for shielding
the amplifier. The shielding should be connected to the amplifier's ground, for example, to the
screws of the cover.