Multichannel Systems MEA1060-Up-BC Manual - Manual (Page 33)

Troubleshooting
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5.5 Stimulation
Recommended stimulus protocols for MEA electrodes
MCS recommends to use only biphasic voltage pulses with the negative phase first. The
duration of the pulse should be 100 μs for each phase and the amplitude between 100 mV
and 3000 mV. Make sure that the voltage level of the stimulating electrode is zero at the
end of the pulse. (See also Potter, S. M., Wagenaar, D. A. and DeMarse, T. B. (2005). Closing
the Loop: Stimulation Feedback Systems for Embodied MEA Cultures. Advances in Network
Electrophysiology Using Multi-Electrode Arrays. M. Taketani and M. Baudry, Springer;
Wagenaar, D. A., Madhavan, R., Pine, J. and Potter, S. M. (2005). "Controlling bursting
in cortical cultures with closed-loop multi-electrode stimulation." J Neurosci 25(3): 680-8.)
Stimulation with MEA electrodes
You can apply two different stimulus protocols to any selection of electrodes. Use the MEA_Select
program to select electrodes for recording and stimulation. The stimulation is triggered with a TTL
pulse, the so-called blanking signal. Please see "Blanking" for more information how to set up
and time the blanking signal.
You can also stimulate without blanking, for example, for testing your setup. Under Blanking,
deselect the Blanking option for disabling the blanking function.