Environmental Express SPE-Express with Cover - Manual (Page 22)

SPE-­‐Express
Operation
and
Instruction
Manual
www.envexp.com 21
2.3.6.6 Method Parameter Specifications
Status Name Input Limits Description
Pre-Rinse Hexane Pre-Rinse Volume 10-20 mL Cleans system below sample bottle with specified amount of
hexane, allows hexane to soak and then siphons hexane to hexane
waste container.
Hexane Pre-Rinse Soak Time 30-120 seconds
Hexane Siphon Time 60-120 seconds
Number of Hexane Pre-Rinses 1-5 rinses
Disk Activation Methanol Injection Volume 10-20 mL Injects desired amount of methanol onto the filter and allows
methanol to soak into the filter.
Methanol Soak Time 60-120 seconds
Sample Filtration Filter Dry Time 10-20 minutes After the sample bottle has drained, the time the filter is allowed to
dry with vacuum prior to elution.
Hexane Rinse Parameters Spray Volume 3.1-30 mL/spray Volume of hexane used to spray the sample bottle.
Sprays per Rinse Cycle 1-10 sprays Number of sprays per rinse cycle
Total Spray Volume 9-30 mL Total volume of hexane used to spray the sample bottle during 1
rinse cycle. *Automatically updates to show the total hexane usage
during the bottle rinses based on the Total Spray Volume and the
Number of Rinse Cycles.
Drain Time 30-120 seconds Amount of time between each rinse cycle.
Number of Rinse Cycles 1-5 rinses The number of rinse cycles the system completes during elution.
Total Volume 27-120 mL The total volume of hexane used during the elution steps.
*Automatically updates to show the total hexane usage during the
bottle rinses based on the Total Spray Volume and the Number of
Rinse Cycles.
Vacuum Parameters Vacuum ON Time 10-20 seconds Pulsing ON/OFF of the vacuum within the glass chamber to remove
eluted hexane from the filter.
Vacuum OFF Time 5-20 seconds
Total Vacuum Cycles 5-10 cycles
Vacuum Dry Time 60-180 seconds Final vacuum duration to pull all remaining hexane into the glass
chamber.
Nitrogen Purge 3-120 seconds Application of nitrogen to push all hexane from the glass chamber
to the evaporation pan.
*automatically calculated
*automatically calculated