Metrohm 940 Professional IC Vario ONE/Proep 2 - Manual (Page 86)

5.15 Separation column
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940 Professional IC Vario ONE/Prep 2 (2.940.1120)
5.15 Separation column
5.15.1 Separating efficiency
The analysis quality that can be attained depends in a large part on the
separating efficiency of the separation column being used. The separation
efficiency of the selected separation column must be sufficient for the cur-
rent analysis problems. If difficulties arise, start by checking the quality of
the separation column in each case by recording a standard chromato-
graph.
You can find detailed information on the separation columns available
from Metrohm in the leaflet provided along with your separation column,
in the Metrohm IC Column Program (available from your Metrohm
representative) or on the Internet at
http://www.metrohm.com in the Ion
Chromatography product area. You can find information on special IC
applications in the corresponding "Application Bulletins" or "Applica-
tion Notes", which are available on the Internet at
http://
www.metrohm.com in the Applications area or free of charge upon
request from your responsible Metrohm representative.
5.15.2 Protecting the separation column
We recommend using the following protective measures so that the sepa-
ration column retains its separating efficiency for as long as possible:
￭ Microfilter both the sample and the eluent (0.45 µm filter) and aspirate
the eluent via the aspiration filter (6.2821.090) as well.
￭ Always use a guard column. Information regarding which guard col-
umn is suitable for your separation column can be found in the
Metrohm IC Column Program (which is available from your
Metrohm representative), the leaflet provided along with your separa-
tion column, the product information about the separation column at
http://www.metrohm.com (Ion Chromatography product area), or
obtained directly from your representative.
￭ Always use the pulsation absorber.
5.15.3 Storing the separation column
Always store separation columns you do not need in a sealed and filled
state according to the column manufacturer's specifications.
5.15.4 Regenerating the separation column
The separation column can be regenerated according to the column man-
ufacturer's specifications if the separation characteristics of the column
have deteriorated. You can find information on regenerating separation
columns available from Metrohm on the leaflet provided with every col-
umn.