Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 10)

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2.2 Lower and Upper Buffer Chamber
The lower buffer chamber forms a stable base for the unit. It houses the an ode and contains the lower
electrophoresis buffer. The upper buffer chamber holds the up per electrophoresis buffer and the elu tion
buffer, and houses the cathode.
Fig. 2. Upper and lower buffer chamber. Lower buffer chamber: Stopcock for lower elec trophoresis buffer inlet (1), and anode (2).
Upper buffer chamber: Elution buffer reservoir (3), upper electrophoresis buffer reservoir (4), ring nut (5), cooling buffer line (6), cooling
buffer feedline (7), and elution buffer feedline (8).
2.3 Cooling Core
The cooling core extends to the bottom of the graduated gel tube and houses the elu tion tube in its center.
Cooling is recommended during polymeriza tion as well as during electrophoresis.
Fig. 3. Cooling core. Cooling buffer ports (1), elution buffer outlet (2), cooling core place ment guides (3), and glazed ceramic cooling
core (4).
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