Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 7)

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1.3 Specifications
Construction
Upper buffer chamber acrylic
Lower buffer chamber acrylic
Electrodes platinum, 0.010 inch diameter
Lid acrylic
Gel tube assembly
glass/acrylic
Elution chamber base acrylic
Elution frit polyethylene
Support frit polyethylene
Cooling core glazed alumina
Elution tube borosilicate glass, 0.06” ID
Casting stand acrylic
Shipping weight 6 lb
Overall size 7 in. diameter x 14 in. high
Voltage limit 500 volts
Current limit 40 milliamperes
Power limit 20 watts
Cooling buffer flow rate 100 ml/min
Elution buffer flow rate 60 ml/hour
Upper electrophoresis buffer volume 300–600 ml
Elution buffer chamber volume 900 ml
Lower electrophoresis buffer volume 2–3 L
1.4 Chemical Compatibility
The Model 491 prep cell is not compatible with chlorinated hydrocarbons (e.g. chlo roform), aromatic
hydrocarbons (e.g. toluene, ben zene), or acetone. Their use will void all warranties.
1.5 Safety
Power to the Model 491 prep cell is to be supplied by an external DC power supply. This power
supply must be ground isolated in such a way that the DC voltage out put floats with respect to
ground. The recommended power sup ply for this instru ment is the PowerPac HV power supply.
The PowerPac universal power supply may also be used. The maximum speci fied operating
parameters for the Model 491 prep cell are:
500 VDC maximum operating voltage
40 mA maximum operating current
20 W maximum operating power limit
Current to the Model 491 prep cell, provided from the external power supply, enters the unit
through the lid assembly, providing a safety interlock to the user. Current flow to the cell is
broken when the lid assembly is removed. Do not attempt to cir cumvent this safety interlock,
and always turn the power supply off when working with the cell.
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