Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 18)

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3. Fill the upper electrophoresis buffer reservoir (300–600 ml) and the elution buffer reservoir (750 ml) and
check that all the lines are properly connected. If leaking is ob served, check connections and reconnect
where necessary.
4. Thoroughly degas 1,500 ml electrophoresis/elution buffer. With the stop cock of the lower buffer tank closed,
fill the lower buffer reservoir with buffer to cover at least the height of gel. The level of lower electrophoresis
buffer should be at least 1–2 cm above the level of the gel poured within the tube. Place the whole gel tube
assembly into the lower buffer reservoir. See Sections 7 and 9 for buffer recipes.
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