Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 42)

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Preparing Ornstein-Davis Native Electrophoresis Buffer Solutions
A. Resolving (Separating) Gel Buffer (1.5 M Tris-HCl pH 8.8)
Dissolve 27.23 grams Tris base in approximately 80 ml deionized water.
Adjust to pH 8.8 with 6 N HCl.
Make to 150 ml with deionized water and store at 4˚C.
B. Stacking Gel Buffer (0.5 M Tris-HCl, pH 6.8)
Dissolve 6 grams Tris base in approximately 60 ml deionized water.
Adjust to pH 6.8 with 6 N HCl.
Make to 100 ml with deionized water and store at 4 ˚C.
C. Sample Buffer (0.0625 M Tris-Cl, pH 6.8, 10% Glycerol, 0.025% Bromophenol Blue)
Deionized water - 5.8 ml
0.5 M Tris-HCl, pH 6.8 - 1.0 ml
Glycerol - 0.8 ml
0.5% Bromophenol blue (optional) - 0.4 ml
Total volume - 8.0 ml
D. 10x Electrode Running Buffer, pH 8.3 (Makes 1 L)
Tris base - 30.3 g
Glycine - 144.0 g
Dissolve and adjust to 1000 ml with deionized water. DO NOT adjust pH with acid or base.
E. To make 1x Electrode Running Buffer (6 L; 0.025 M Tris, 0.192 M Glycine, pH 8.3)
Dilute 600 ml 10x electrophoresis buffer (pH 8.3) with 5,400 ml deionized wa ter. Do not adjust
pH with acid or base.
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