Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 17)

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4. To simplify sample loading, the sample loading guide should be inserted into the space between the cooling
core and the gel tube at this time.
3.4 Assembly of Upper and Lower Buffer Chambers
1. Carefully place the upper buffer reservoir on the gel tube assembly and seat it firmly. Make sure the small
O-ring is properly seated in the threaded con nector.
The following connections should be made before the black ring nut is tightened. Align and connect the
elution buffer feedline (white cap) to the elution buffer reser voir and to the elution port (white port) on the
elution chamber. Then connect the recirculation buffer feedline (clear cap) to the port at the bottom of
the upper elec trophoresis buffer reservoir and to the recirculation buffer port on the elution chamber
(clear port).
2. Once the feedlines from the upper buffer chamber are aligned and secured to the connectors on the
elution chamber, hand tighten the black ring nut. This procedure will prevent attempting to turn the
chamber against the tightened nut. Connect the cooling buffer line (clear cap) already attached inside
the upper elec trophoresis reservoir with one of the two clear ports on the top of the cooling core to form
a small loop. Secure the second cooling buffer line (clear cap) to the other clear port on the cooling core;
this line connects to the inlet of the buffer recirculation pump. Connect the small diameter elution tubing
to the white elution buffer outlet in the center of the cooling core. Guide both the recirculating buffer line
and the elution tubing from the cooling core through the notch at the perimeter of the up per buffer
chamber.
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