Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 34)

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7.4 10x Electrode (Running) Buffer, pH 8.3
(Makes 1 L)
Tris base 30.3 g
Glycine 144.0 g
SDS 10.0 g
Dissolve and adjust to 1,000 ml with deionized water. DO NOT adjust pH with acid or base.
To make 6 L of 1x electrophoresis buffer (0.025 M Tris, 0.192 M glycine, 0.1% SDS, pH 8.3) for the prep cell,
dilute 600 ml of 10x stock with 5,400 ml deionized wa ter.
7.5 30% Acrylamide Stock Solution
Acrylamide/bis (N,N'-bis-methylene-acry lamide)
(30%T/2.67%C)
Acrylamide 146.0 g
Bis 4.0 g
Dissolve in about 350 ml deionized water then adjust to 500 ml with deion ized wa ter. Filter and store at 4°C
in the dark.
(Preweighed acrylamide/bis 37.5:1 mixture or 40% acrylamide/bis stock so lution can be substituted.
Calculate the volume of a 40% stock solution re quired to make the desired gel in Section 8.2).
Caution: Acrylamide monomer is a neurotoxin.
Avoid breathing acrylamide dust, do not pipet acrylamide solutions by mouth, and wear gloves when
handling acrylamide powder or solutions con taining it. For dis posal of unused acrylamide, add bis-
acrylamide (if none is present), induce polymer ization, and discard the solidified gel.
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