Bio-Rad Bio-Plex Pro™ Human Chemokine Assays - Manual (Page 10)

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1. Plan Plate Layout
Determine the total number of wells in the experiment using the Plate
Layout Template on page 35 or the Plate Formatting tab in
Bio-Plex Manager
™ . A suggested plate layout is shown in Figure 2,
with all conditions in duplicate.
1. Assign standards to columns 1 and 2, with the highest concentration
in row A and the lowest concentration in row H.
2. Assign the blank to wells A3 and A4. The blank should consist of your
chosen standard diluent. Note that Bio-Plex Manager automatically
subtracts the blank (B) MFI value from all other assay wells.
3. User-specified controls, as well as the quality controls supplied in
premixed kits, are assigned to wells in columns 3 and 4.
4. The remainder of the plate is available for samples.
5. once the total number of wells is known, you can calculate the
required volumes of beads, detection antibody, and streptavidin-PE.
Use Tables 6–7, 9–10, and 11, respectively, or the Calculation
Worksheet on pages 36–37.
Legend
S Standard
B Blank
X Samples
C Controls
Fig. 2. Suggested plate layout. For detailed instructions on plate
formatting in Bio-Plex Manager, see the Read Plate section.