Bio-Rad Bio-Plex Pro™ Human Chemokine Assays - Manual (Page 13)

11
Setting up a Vacuum Manifold
Calibrate the vacuum manifold by placing a standard 96-well flat bottom
plate on the unit and adjusting the pressure to –1 to –3" Hg. In general,
100 µl liquid should take 3–4 sec to clear the well. For more detailed
instructions, refer to bulletin #10005042.
4. Prepare Wash Buffer
1. Bring the 10x stock solution to room temperature.
2. If crystals exist, ensure that they are completely dissolved. Mix the
10x stock solution by inversion before preparing the 1x wash buffer.
3. To prepare 1x wash buffer, dilute 1 part of 10x stock solution with
9 parts of deionized water.
5. Prepare Standards and Controls
General Instructions
n
It is essential to prepare standards and quality controls (if included)
exactly as described in this section. Incorrect preparation may lead to
low signal or variable measurements from plate to plate
n
The product data sheet provided with the standards lists the most
concentrated point on the standard curve (S1). Enter this information
into Bio-Plex Manager
™ software as instructed in section 9
Using the Quality Controls (optional)
A single vial of quality controls is provided with the 40-plex fixed panel only.
Their use is intended for monitoring the day-to-day quality of assay results.