Bio-Rad Bio-Plex Pro™ Human Chemokine Assays - Manual (Page 28)

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Acquire Data
1. Shake the assay plate at 850 ± 50 rpm for 30 sec, and visually
inspect the plate to ensure that the assay wells are filled with buffer.
Slowly remove the sealing tape and any plate cover before placing
the plate on the plate carrier.
2. Click Run Protocol and on the pop-up screen, select Load Plate
and click OK to start acquiring data.
3. Use the Wash Between Plates command after every plate run
to reduce the possibility of clogging the instrument.
4. If acquiring data from more than one plate, empty the waste bottle and
refill the sheath bottle after each plate (if HTF are not present). Select
Wash Between Plates and follow the instructions. Then repeat the
Prepare Protocol and Acquire Data instructions.
5. When data acquisition is complete, select Shut Down and
follow the instructions.
Reacquire Data
It is possible to acquire data from a well or plate a second time using the
Rerun/Recovery mode located below Start in the Run Protocol step.
Any previous data will be overwritten.
1. Check the wells from which data will be reacquired.
2. Aspirate the buffer with the wash method of choice, but do not
perform the wash step.
3. Add 100 µl of assay buffer to each well. Cover the plate with a new
sheet of sealing tape. Shake the plate at 850 ± 50 rpm for 30 sec.
Slowly remove the sealing tape before placing the plate on the
plate reader.
4. Repeat the Acquire Data steps to reacquire data. The data acquired
should be similar to those acquired initially; however, the acquisition
time will be extended because the wells have fewer beads.